Thermostable chimeric PQQ glucose dehydrogenase
نویسندگان
چکیده
منابع مشابه
A Disposable Electrochemical Glucose Sensor Using Catalytic Subunit of Novel Thermostable Glucose Dehydrogenase
We report here the development of a novel disposable-type glucose enzyme sensor based on a screen-printed carbon electrode (SPCE) employing a catalytic subunit of FAD-dependent glucose dehydrogenase (FADGDH) and a Rucomplex (hexaammineruthenium (III) chloride) as the electron mediator. The whole blood glucose measurement was achieved in 2 seconds using a 500 nl sample of the range of 100-800 mg...
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BACKGROUND Self-monitoring of blood glucose (SMBG) is an important component of the modern therapy for diabetes mellitus. Thanks to the current progress in electronics and sensor fabrication technology, both the time and the blood sample volume required for the measurement have decreased drastically. However, devices that work with an even smaller sample volume and a shorter measurement time ar...
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A novel potential treatment technique applied to a glucose biosensor that is based on pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenase (GDH) and chromium hexacyanoferrate (CrHCF) incorporated into a platinum (Pt) electrode was demonstrated. CrHCF, serving as a mediator, was electrochemically deposited on the Pt electrode as ascertained by CV, SEM, FTIR and XPS measurements. The po...
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An engineered Escherichia coli PQQ glucose dehydrogenase (PQQGDH) with improved enzymatic characteristics was constructed by substituting and combining the gene-encoding protein regions responsible for EDTA tolerance, thermal stability and substrate specificity. The protein region responsible for complete EDTA tolerance in Acinetobacter calcoaceticus, which is recognized as the indicator of hig...
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Background: Jaundice is affecting over 60-80 percent of neonates in the first week of life. Glucose-6-phosphate dehydrogenase (G6PD) deficiency, which is an important cause of pathologic hyperbilirubinemia, can lead to hemolytic anemia, jaundice and kernicterus. The present study was performed to determine the prevalence of G6PD deficiency among icteric neonates in Shirvan, Iran. Methods: This...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1995
ISSN: 0014-5793
DOI: 10.1016/0014-5793(95)00418-9